diff --git a/src/Mixins/Expectation.php b/src/Mixins/Expectation.php
index 2617fea4..c1ad83dc 100644
--- a/src/Mixins/Expectation.php
+++ b/src/Mixins/Expectation.php
@@ -16,6 +16,7 @@ use Pest\Matchers\Any;
 use Pest\Support\Arr;
 use Pest\Support\Exporter;
 use Pest\Support\NullClosure;
+use Pest\Support\Str;
 use Pest\TestSuite;
 use PHPUnit\Framework\Assert;
 use PHPUnit\Framework\Constraint\Constraint;
@@ -1111,4 +1112,20 @@ final class Expectation
 
         return $this;
     }
+
+    /**
+     * Asserts that the value is UUID.
+     *
+     * @return self<TValue>
+     */
+    public function toBeUUID(string $message = ''): self
+    {
+        if (! is_string($this->value)) {
+            InvalidExpectationValue::expected('string');
+        }
+
+        Assert::assertTrue(Str::isUuid($this->value), $message);
+
+        return $this;
+    }
 }
diff --git a/src/Support/Str.php b/src/Support/Str.php
index ee4e7231..3c9bb881 100644
--- a/src/Support/Str.php
+++ b/src/Support/Str.php
@@ -92,4 +92,12 @@ final class Str
     {
         return $search === '' ? $subject : array_reverse(explode($search, $subject, 2))[0];
     }
+
+    /**
+     * Determine if a given value is a valid UUID.
+     */
+    public static function isUuid(string $value): bool
+    {
+        return preg_match('/^[\da-f]{8}-[\da-f]{4}-[\da-f]{4}-[\da-f]{4}-[\da-f]{12}$/iD', $value) > 0;
+    }
 }
diff --git a/tests/Features/Expect/toBeUUID.php b/tests/Features/Expect/toBeUUID.php
new file mode 100644
index 00000000..c77d6bb4
--- /dev/null
+++ b/tests/Features/Expect/toBeUUID.php
@@ -0,0 +1,31 @@
+<?php
+
+use Pest\Exceptions\InvalidExpectationValue;
+use PHPUnit\Framework\ExpectationFailedException;
+
+test('failures with wrong type', function () {
+    expect([])->toBeUUID();
+})->throws(InvalidExpectationValue::class, 'Invalid expectation value type. Expected [string].');
+
+test('pass', function () {
+    expect('3cafb226-4326-11ee-a516-846993788c86')->toBeUUID(); // version 1
+    expect('0000415c-4326-21ee-a700-846993788c86')->toBeUUID(); // version 2
+    expect('3f703955-aaba-3e70-a3cb-baff6aa3b28f')->toBeUUID(); // version 3
+    expect('ca0a8228-cdf6-41db-b34b-c2f31485796c')->toBeUUID(); // version 4
+    expect('a35477ae-bfb1-5f2e-b5a4-4711594d855f')->toBeUUID(); // version 5
+    expect('1ee43263-cf5a-6fd8-8f47-846993788c86')->toBeUUID(); // version 6
+    expect('018a2bef-09f2-728c-becb-c3f569d91486')->toBeUUID(); // version 7
+    expect('00112233-4455-8677-8899-aabbccddeeff')->toBeUUID(); // version 8
+});
+
+test('failures', function () {
+    expect('foo')->toBeUUID();
+})->throws(ExpectationFailedException::class);
+
+test('failures with message', function () {
+    expect('bar')->toBeUUID('oh no!');
+})->throws(ExpectationFailedException::class, 'oh no!');
+
+test('not failures', function () {
+    expect('foo')->not->toBeUUID();
+});